Symposia and Oral Sessions

Although the BovineSNP50 BeadChip (Illumina, Inc.) was developed for genomic selection, the results can also be used to validate paternity, to determine the genotyping error rate, the minimum number of SNPs required for paternity validation, and to delete defective SNPs from further analysis. Of 576 Israeli Holstein bulls genotyped, the sire was also genotyped for 204 bulls born between 1982 and 2003. Thirteen % of the genotypes were deleted due to call rates < 0.7. SNPs were deleted from this analysis if < half the bulls had valid genotypes, minor allele frequency < 0.05, the SNP was not assigned to an autosome, or the absolute value of the deviation of the observed frequency of heterozygotes from the expected Hardy-Weinberg frequency > 0.2. Of the 54001 SNPs included on the BeadChip, 14,960 (28%) were deleted. For diallelic markers with only a single parent genotyped, a discrepancy with Mendelian inheritance is obtained only if the two individuals are homozygous for different alleles. In addition to analysis with all SNPs, paternity was verified based on each hundredth SNP (388 SNPs) and each four-hundredth SNP (97 SNPs). Paternity was incorrectly recorded for 7 bulls (3.5%). In the analysis of 388 SNPs, the number of discrepancies for these 7 bulls with their putative sire was > 15 with a minimum frequency of > 0.04, while for all other bulls the frequency of discrepancies was < 3 for a maximum frequency of < 0.016. Of the 108 bulls born before 1997, paternity was excluded in 6 cases (5.5%), while for the 96 bulls born since 1997 there was only a single exclusion. LB1 Putative in utero epigenetic impacts of dam lactation yield and tissue energy stores on daughter first lactation milk production in dairy cattle. R. A. Erdman*1, J. A. Arias2, E. Quinn-Walsh2, P. Fisher3, K. Stelwagen4, and K. Singh4, 1University of Maryland, College Park, 2Livestock Improvement Corporation, Hamilton, New Zealand, 3AgResearch Ltd, Invermay Agricultural Centre, Mosgiel, New Zealand, 4AgResearch Ltd, Ruakura Research Centre, Hamilton, New Zealand.